Lipids & metabolic liver disease
Hepatic and adipose tissue transcriptome analysis highlights a commonly deregulated autophagic pathway in severe MASLD
Authors' conclusion
By comparing the hepatic and VAT transcriptome during MASLD, we refined the concept by which CTSD may represent a potential biomarker of severe disease.
Study context
- Population
- Patients with obesity with MASLD; hepatic and visceral adipose tissue (VAT) samples79 matched VAT samples
- Participants
- 167 hepatic samples
- Comparison
- Severe vs mild MASLD
- Outcome
- Hepatic and VAT transcriptome pathways in severe vs mild MASLD; CTSD as diagnostic marker of severe MASLD
Methodological detailsRNA sequencing of hepatic and VAT samples; circulating cathepsin D (CTSD) by ELISA; autophagy-lysosomal pathway by Western blot (n = 20)
- RNA sequencing of hepatic and VAT samples; circulating CTSD by ELISA; autophagy-lysosomal pathway by Western blot (n = 20)
Main finding
As reported in the abstract
Deregulated genes in tissue comparative analysis, including CTSD, which showed the most robust diagnostic accuracy in discriminating mild and severe MASLD
13
CTSD expression correlated with circulating protein; increase validated in histologically characterized MASLD patients, with high accuracy in foreseeing severe liver injury
432 patients
Further findings
Serum CTSD validation in histologically characterized MASLD patients
- Circulating CTSD increase validated, with high accuracy in foreseeing severe liver injury; n
- 432
- Serum CTSD increased the performance of fibrosis 4 in diagnosing advanced disease
Original publication
Hepatic and adipose tissue transcriptome analysis highlights a commonly deregulated autophagic pathway in severe MASLD. Obesity (Silver Spring). Published 4 March 2024. doi:10.
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