Expression of Neurotensin and Its Receptors Along the Intestinal Tract in Type 2 Diabetes Patients and Healthy Controls
Authors' conclusion
This unique intestinal mapping of N cell density and NTS expression shows increasing levels from the small intestine's proximal to distal end (without differences between patients with T2D and healthy controls), while negligible N-cells and NTS mRNA expression were observed in the large intestine. Sortilin was expressed throughout the intestines in both groups; no NTS receptor 1 or 2 mRNA expression were detected.
Study context
- Population
- Patients with T2D and matched healthy controls; mucosa biopsies along the entire small intestine (30-cm intervals) and 7 locations in the large intestine
- Participants
- 12 vs 12
- Comparison
- T2D vs healthy controls
- T2Dn = 12
- Healthy controlsn = 12reference
- Outcome
- N cell density and NTS/NTS receptor mRNA expression along the intestine
Methodological detailsmatched healthy controls (sex, age, BMI), double-balloon enteroscopy, immunohistochemistry and messenger RNA (mRNA) sequencing
- Matched healthy controls (sex, age, BMI)
- Double-balloon enteroscopy
- Immunohistochemistry and mRNA sequencing
Main finding
As reported in the abstract
- N cell density and neurotensin (NTS) mRNA expression gradually increased from duodenum to ileum
- Negligible NTS-positive cells and NTS mRNA expression in the large intestine
- NTS receptor 1 and 2 mRNA expression not detected
- Sortilin uniformly expressed in the intestines
- T2D had lower NTS-positive cells and mRNA expression than healthy controls, not statistically significant after adjusting for multiple testing
Original publication
Expression of Neurotensin and Its Receptors Along the Intestinal Tract in Type 2 Diabetes Patients and Healthy Controls. J Clin Endocrinol Metab. Published 14 March 2023. doi:10.
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