Validation of a novel approach to assess metabolic flexibility to a high‐fat meal in a whole‐body room calorimeter
Authors' conclusion
Overnight changes in substrate oxidation following a high-fat dinner meal represent a unique aspect of MetFlex that cannot be captured using more conventional methods. Our findings warrant prospective studies to determine whether these parameters are predictive of the development of obesity or metabolic dysfunction.
Study context
- Population
- Healthy adults, predominantly White and male; mean (SD) age 29.4 (6.3) years, BMI 25.4 (4.1) kg/m²
- Participants
- 8 adults
- Comparison
- Metabolic flexibility (MetFlex) to a high-fat meal vs MetFlex to carbohydrates
- Outcome
- Test-retest reliability of MetFlex to fat and correlation with clamp-measured MetFlex
Methodological detailstest-retest reliability, two overnight metabolic chamber stays, two-step hyperinsulinemic-euglycemic clamp comparison, MetFlex to fat: two overnight metabolic chamber stays 5 to 7 days apart, MetFlex to carbohydrates: two-step hyperinsulinemic-euglycemic clamp >5 days later
- Test-retest reliability
- Two overnight metabolic chamber stays
- Two-step hyperinsulinemic-euglycemic clamp comparison
- MetFlex to fat: two overnight metabolic chamber stays 5 to 7 days apart
- MetFlex to carbohydrates: two-step hyperinsulinemic-euglycemic clamp >5 days later
Performance
As reported in the abstract
- MetFlex to fat: satisfactory test-retest reliability for several outcomes (intraclass correlation coefficient > 0.70)
- No correlation of MetFlex to fat with MetFlex measured during the clamp
Original publication
Validation of a novel approach to assess metabolic flexibility to a high‐fat meal in a whole‐body room calorimeter. Obesity (Silver Spring). Published 6 March 2025. doi:10.
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