The RCAN1.4 Metastasis Suppressor Is Hypermethylated at Intron 1 in Thyroid Cancer
Authors' conclusion
The MPS gene, RCAN1.4, is downregulated in thyroid cancer cells and human thyroid cancer in part by hypermethylation of CG-rich regions in intron 1.
Study context
- Population
- 5 thyroid cancer cell lines (TPC1, FTC133, BCPAP, C643, 8505C) with different genetic drivers and 18 paired normal and thyroid cancer human tissues
- Participants
- 18 paired tissues
- Comparison
- Thyroid cancer vs paired normal thyroid tissue; cell lines with vs without decitabine
- Outcome
- RCAN1.4 expression and methylation
Main finding
As reported in the abstract
- RCAN1.4 messenger RNA (mRNA) and protein increased after decitabine in all assessed cell lines
- 3 cytosine-guanine (CG)-rich regions identified in silico: 1 in proximal promoter, 2 in intron 1
- Hypermethylation of intron 1 CG-rich regions identified by capture method and methylation-specific polymerase chain reaction (MSPCR)
- No hypermethylation of proximal promoter CG-rich region
- Intron 1 hypermethylation in cancer samples associated with lower RCAN1.4 mRNA
- Increased NFE2L3 expression in cancer samples, a downstream marker of RCAN1.4 loss
Original publication
The RCAN1.4 Metastasis Suppressor Is Hypermethylated at Intron 1 in Thyroid Cancer. Thyroid. Published 15 May 2023. doi:10.
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