Single-cell RNA sequencing reveals that an imbalance in monocyte subsets rather than changes in gene expression patterns is a feature of postmenopausal osteoporosis
Authors' conclusion
The scRNA-seq findings suggest that an imbalance in monocyte subsets is a characteristic feature of PMOP.
Study context
- Population
- Postmenopausal women; CD14+ bone marrow monocytes analysed by single-cell RNA sequencing (scRNA-seq)
- Participants
- 3 vs 3
- Comparison
- Women with postmenopausal osteoporosis (PMOP) vs postmenopausal women with normal BMD
- PMOPn = 3
- Normal BMDn = 3reference
- Outcome
- Monocyte subset proportions (7 clusters)
Methodological detailssingle-cell RNA sequencing, bulk transcriptome validation (GEO), flow cytometry validation
- Single-cell RNA sequencing
- Bulk transcriptome validation (GEO)
- Flow cytometry validation
Main finding
As reported in the abstract
- Proportion of cluster 1 significantly decreased in PMOP
- Proportion of cluster 7 increased in PMOP
- Cluster 7: upregulated inflammation, immunity and osteoclast differentiation pathways; cluster 1 the opposite
- Cluster 1 the initial subset; cluster 7 one of the terminal subsets
- CD16 marker of cluster 7; increased proportion of CD16+ monocytes in PMOP
Further findings
Validation in bulk transcriptome data (GEO)
- Altered proportions of clusters 1 and 7 validated and of diagnostic significance
Additional scRNA-seq of bone marrow mononuclear cells (3 PMOP, 3 normal BMD)
- Differential proportions of clusters 1 and 7 confirmed again
Original publication
Single-cell RNA sequencing reveals that an imbalance in monocyte subsets rather than changes in gene expression patterns is a feature of postmenopausal osteoporosis. J Bone Miner Res. Published 23 April 2024. doi:10.
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