In-depth Microbiological Characterization of Urine From Subjects With Type 2 Diabetes
Authors' conclusion
The urinary microbiome of subjects with T2D was found to be different from that of healthy controls. Specifically, patients with T2D displayed higher total bacterial load and Bacillota levels, as well as qualitative changes in bacterial species. These changes suggested a dysbiotic condition of the urinary microbiota of subjects with T2D, with some gender-related differences. Although causality cannot be inferred, these findings highlight the impact of T2D on the urinary microbiota and its potential relevance in developing LUTS and, from a broader perspective, metabolic abnormalities.
Study context
- Population
- Patients with T2D without lower urinary tract symptoms (LUTS) and healthy individuals
- Participants
- 50 vs 25
- Comparison
- T2D vs healthy controls
- T2Dn = 50
- Healthy controlsn = 25reference
- Outcome
- Urinary microbiota (qPCR, 16S rRNA sequencing)
Methodological detailspilot study, Microbial DNA from urinary sediments; quantitative polymerase chain reaction (PCR), 16S rRNA gene sequencing; validation with digital PCR
- Pilot study
- Microbial DNA from urinary sediments; quantitative PCR, 16S rRNA gene sequencing; validation with digital PCR
Main finding
As reported in the abstract
- Higher total bacterial load and increased abundance of Bacillota in T2D; after stratification by gender, observed only in women
- No significant quantitative differences at the genus level
- Alpha diversity: no significant differences between T2D and controls, or by gender
- Species level: substantial qualitative and often gender-dependent shift in T2D
Observational data: associations, not effects.
Original publication
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