In-depth analysis of metabolic hormones and inflammatory markers following Roux-en-Y gastric bypass in humans and rodents: similarities and differences
Authors' conclusion
Preoperative GLP-1 levels in humans correlate with weight loss after RYGB and could potentially be predictive. The investigated rat model shows largely comparable patterns of incretins and adipokines. Given its physiological similarity, this model is suitable for testing pharmacological agents that mimic anorexigenic hormones, potentially guiding novel treatments for severe obesity.
Study context
- Population
- Humans from the WAS trial (fasting plasma at baseline and 12 months) and male Wistar rats with diet-induced obesity (7 weeks after surgery)
- Participants
- 20 / 17 / 12 / 12
- Comparison
- RYGB vs controls and RYGB vs sham surgery
- RYGB (humans)n = 20
- Controlsn = 17reference
- RYGB (rats)n = 12
- Sham surgeryn = 12reference
- Outcome
- Fasting peptide hormones and cytokines after RYGB, humans vs rats
- Follow-up
- 12 months (humans); seven weeks after surgery (rats)
Methodological detailssecondary analysis of samples from the randomized controlled WAS trial, rat model of diet-induced obesity with sham surgery control, 18 peptide hormones and 21 amino acids measured by magnetic multiplex assays, ELISA and LC-MS/MS
- Secondary analysis of samples from the randomized controlled WAS trial
- Rat model of diet-induced obesity with sham surgery control
- 18 peptide hormones and 21 amino acids measured by magnetic multiplex assays, ELISA and LC-MS/MS
Main finding
As reported in the abstract
12 months (humans); seven weeks after surgery (rats)
- GLP-1 and PYY3-36 significantly lower in humans after RYGB
- both p < 0.001
- Fasting insulin lower after RYGB in humans
- p < 0.001
- Fasting insulin lower after RYGB in rats
- p < 0.01
- Leptin significantly lower after RYGB in humans
- p < 0.001
- Leptin significantly lower after RYGB in rats
- p < 0.05
- IL-6 and MCP-1 significantly lower in humans after RYGB
- p < 0.01, p < 0.05
Further findings
Correlation of pre-RYGB GLP-1 with weight change after 12 months (humans)
- Positive correlation, Pearson's r
- 0.733
- P
- P < 0.05
Correlation of leucine with GLP-1 12 months after RYGB
- Humans: positive correlation, Pearson's r
- 0.588
- Humans: P
- P < 0.05
- Rats: no correlation
Original publication
In-depth analysis of metabolic hormones and inflammatory markers following Roux-en-Y gastric bypass in humans and rodents: similarities and differences. Diabetes Res Clin Pract. Published 24 September 2025. doi:10.
Composed by AI from the published abstract and checked against it. How Briefs are made →