High-Throughput Proteomics and Immunohistochemistry of Orbital Connective Tissue in Graves' Orbitopathy
Authors' conclusion
For the first time, combined proteomics and immunohistochemistry detected upregulated orbital tissue proteins potentially implicated in GO progression.
Study context
- Population
- Orbital connective tissue samples from patients with Graves' orbitopathy (GO) and control subjects
- Participants
- 31 tissue samples
- Comparison
- Severe GO vs mild GO vs controls
- Severe GOn = 11
- Mild GOn = 12
- Controln = 8reference
- Outcome
- DEPs on HTP
Methodological detailshigh-throughput proteomics, immunohistochemical validation
- High-throughput proteomics
- Immunohistochemical validation
Main finding
As reported in the abstract
- Proteins identified
- 4579
- Differentially expressed proteins (DEPs), mild GO vs control (P < .05)
- 847
- DEPs, severe GO vs control (P < .05)
- 790
- DEPs, severe vs mild GO (P < .05)
- 208
- Upregulated DEPs (fold change ≥2.0) overlapping between severe GO and both mild GO and control: LTBP2, lysyl oxidase homolog, Ras-interacting protein 1, integrin beta 3, coagulation factor XII
- 5
- Upregulated DEPs involved in angiogenesis, inflammation, tissue remodeling and fibrosis
- 163
Further findings
Immunohistochemical validation (11 severe, 7 mild GO tissues)
- IHC tissue validation confirmed the high-throughput proteomics (HTP) findings
Original publication
High-Throughput Proteomics and Immunohistochemistry of Orbital Connective Tissue in Graves' Orbitopathy. J Clin Endocrinol Metab. Published 21 May 2025. doi:10.
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