M1-Like Macrophages Modulate Fibrosis and Inflammation of Orbital Fibroblasts in Graves' Orbitopathy: Potential Relevance to Soluble Interleukin-6 Receptor
Authors' conclusion
Our study demonstrated the immunophenotypes of orbital-infiltrating macrophages that may activate OFs depending on the IL-6/sIL-6R signaling in GO.
Study context
- Population
- Orbital connective tissues from patients with Graves' orbitopathy and healthy controls
- Participants
- 40 vs 20
- Comparison
- Graves' orbitopathy (GO) vs healthy controls
- GOn = 40
- Healthy controlsn = 20reference
- Outcome
- Macrophage immunophenotypes and effects on orbital fibroblasts (OFs)
Main finding
As reported in the abstract
- GO orbital connective tissues displayed increased IL-6, sIL-6R, STAT3 and IL-17A levels
- CD86+ M1-like macrophages predominant in active GO; stable GO tended to have more CD163+ M2-like macrophages
- IL-6 expression higher in M1-like macrophages, TGF-β higher in M2-like macrophages, in situ in vivo and in vitro
- IL-6/sIL-6R stimulation promoted fibrosis of both CD34+ and CD34- orbital fibroblasts (OFs) and induced MCP-1 expression in both subsets
- IL-6/sIL-6R enhanced antigen processing of CD34+ OFs via upregulation of intact MHC I and antigen transporters
- Thyrotropin receptor and IGF-1 receptor protein expression not directly increased by IL-6/sIL-6R in CD34+ OFs
- sIL-6R was conducive to differentiation of CD8+ IL-17A-producing T cells
Observational data: associations, not effects.
Original publication
M1-Like Macrophages Modulate Fibrosis and Inflammation of Orbital Fibroblasts in Graves' Orbitopathy: Potential Relevance to Soluble Interleukin-6 Receptor. Thyroid. Published 20 February 2023. doi:10.
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