Glucose Load Following Prolonged Fasting Increases Oxidative Stress– Linked Response in Individuals With Diabetic Complications
Authors' conclusion
These findings suggest increased susceptibility to glucose-induced oxidative stress in individuals with diabetes complications after prolonged fasting and might help in diet interventions for diabetes management.
Study context
- Population
- Glucose-tolerant individuals and individuals with T2D with or without diabetes complications undergoing OGTT before and after a 5-day fasting-mimicking diet
- Participants
- 10 / 10 / 10
- Comparison
- T2D with complications vs T2D without complications vs glucose-tolerant controls
- T2D+n = 10
- T2D-n = 10
- CONn = 10reference
- Outcome
- Change in PBMC resistance against ex vivo methylglyoxal (MG)
- Follow-up
- Postglucose load and fasting
Methodological detailsex vivo peripheral blood mononuclear cell (PBMC) methylglyoxal exposure, OGTT before and after 5-day fasting-mimicking diet
- Ex vivo PBMC methylglyoxal exposure
- OGTT before and after 5-day fasting-mimicking diet
Main finding
As reported in the abstract
T2D+
−19.0%
T2D-
−1.7%
CON
12.6%
All P = 0.017
Further findings
| Outcome | P value |
|---|---|
| Mitochondrial biogenesis (mRNA HspA9), T2D- vs CON · postglucose load and fastingIncreased in T2D- | P = 0.032 |
| Antioxidant defenses postfasting (not postglucose load)T2D- vs CON · mRNA forkhead box O4 increased in T2D- | P = 0.036 |
| Antioxidant defenses postfasting (not postglucose load)T2D- vs CON · glutathione-peroxidase-2 increased in T2D- | P = 0.034 |
| Antioxidant defenses postfasting (not postglucose load)T2D- vs T2D+ · glutathione-peroxidase-2 increased in T2D- | P = 0.04 |
Dicarbonyl detoxification, oxidative stress and mitochondrial complex V expression, T2D+ vs T2D- and CON · postglucose load and fasting
- Increased mRNA glyoxalase-1 in T2D+
- all P = 0.039
- Increased mRNA glutathione-disulfide-reductase in T2D+
- all P = 0.006
- Increased mitochondrial complex V protein in T2D+
- all P = 0.004
Mitochondrial number (citrate synthase activity)
- Citrate synthase activity remained unchanged
Original publication
Glucose Load Following Prolonged Fasting Increases Oxidative Stress– Linked Response in Individuals With Diabetic Complications. Diabetes Care. Published 21 June 2024. doi:10.
Composed by AI from the published abstract and checked against it. How Briefs are made →