Feasibility and Validity of In-Home Self-Collected Capillary Blood Spot Screening for Type 1 Diabetes Risk
Authors' conclusion
Self-collected blood spot sampling is preferred over venesection by research participants. ADAP and multiplex ELISA are highly specific assays for islet antibodies in blood spots with acceptable performance for use alone or in combination to facilitate screening for type 1 diabetes risk.
Study context
- Population
- People living with T1D and their family members; 50 antibody-negative (Abneg) and 47 antibody-positive (Abpos)
- Participants
- 97 paired samples
- Comparison
- Blood spot antibody detection by agglutination PCR (ADAP) and multiplex enzyme-linked immunoassay (ELISA) vs serum radioimmunoassay (RIA)/ELISA for islet antibodies
- Outcome
- Detection of Abpos by blood spot
Methodological detailspaired self-collected capillary blood spot and health-worker-collected serum samples, Testable blood spot samples returned by 43 Abneg and 47 Abpos
- Paired self-collected capillary blood spot and health-worker-collected serum samples
- Testable blood spot samples returned by 43 Abneg and 47 Abpos
Performance
As reported in the abstract
| Test or model | Sensitivity | Specificity |
|---|---|---|
| ADAP | 85% | 98% |
| Multiplex ELISA | 87% | 100% |
Sensitivity
ADAP85%
Multiplex ELISA87%
Specificity
ADAP98%
Multiplex ELISA100%
Further findings
- ADAP by each of four antigen specificities
- 98% to 100% · specificity range · AUC range 0.841 to 0.986
Acceptability of self-collection
- Majority found self-collection easier, more convenient and less painful than formal venesection
Original publication
Feasibility and Validity of In-Home Self-Collected Capillary Blood Spot Screening for Type 1 Diabetes Risk. Diabetes Technol Ther. Published 16 January 2024. doi:10.
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