University Exams and Psychosocial Stress: Effects on Cortisol Rhythmicity in Students

Clin Endocrinol (Oxf)Published Pilot observational study

Authors' conclusion

Exam periods may be accompanied by elevated cumulative cortisol exposure while short-term HPA rhythmicity remains preserved. The combined use of hair and salivary biomarkers offers a multidimensional and feasible approach to investigating acute and cumulative stress responses in students. Larger longitudinal studies incorporating behavioural measures are warranted to confirm these findings.

Study context

Population
Undergraduate students aged 18–24 years from a biological sciences programme
Participants
27 students
Exposure
Exam month vs non-exam month
Outcome
Hair cortisol levels

Main finding

As reported in the abstract

  • Hair cortisol significantly higher in November (exam month) than in October (non-exam month)

Observational data: associations, not effects.

Further findings

Salivary cortisol during 3 consecutive exam weekdays · Monday to Wednesday, five daily time points

General diurnal pattern and cortisol awakening response (CAR) across the week
Morning cortisol: nonsignificant numerical increase across days
Daily cortisol output (AUC) remained stable

Original publication

University Exams and Psychosocial Stress: Effects on Cortisol Rhythmicity in Students. Clin Endocrinol (Oxf). Published 17 December 2025. doi:10.1111/cen.70083

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