Senescence markers in subcutaneous preadipocytes differ in childhood‐ versus adult‐onset obesity before and after weight loss
Authors' conclusion
These results provide preliminary evidence that females with CO have an accelerated preadipocyte aging state that improves with weight loss in terms of DNA damage but not senescence.
Study context
- Population
- Females with obesity undergoing diet- and exercise-induced moderate (≈10%) weight loss; abdominal (AB) and femoral (FEM) subcutaneous adipose tissue (SAT)
- Comparison
- Childhood-onset obesity (CO) vs adult-onset obesity (AO), before and after weight loss
- Outcome
- Senescence-related markers (γH2AX/RAD51 DNA damage/repair; p53/p21 senescence; SA-β-gal activity) in AB and FEM SAT
- Follow-up
- Before and after moderate (≈10%) weight loss
Main finding
As reported in the abstract
Senescence-related markers in preadipocytes
- At baseline, CO had proportionately more AB and FEM preadipocytes with DNA damage (γH2AX+) and senescence markers (p53+ and/or p21+) than AO
- Proportion of γH2AX+ FEM preadipocytes declined with weight loss in CO; similar between groups after weight loss
- Number of γH2AX foci in γH2AX+ preadipocytes decreased similarly between groups and regions with weight loss, in parallel with an increase in RAD51
- Proportion of p53+ and p21+ preadipocytes and SA-β-gal+ cells in SAT did not change with weight loss
- Total p21 intensity in p53+/p21+ FEM preadipocytes declined with weight loss in AO
Original publication
Senescence markers in subcutaneous preadipocytes differ in childhood‐ versus adult‐onset obesity before and after weight loss. Obesity (Silver Spring). Published 17 May 2023. doi:10.
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