New liquid chromatography tandem mass spectrometry reference data for estradiol show mini-puberty in both sexes and typical pre-pubertal and pubertal patterns
Authors' conclusion
Variability of RIs for E2 between studies illustrates the importance of laboratory-specific RIs despite using a LC-MS/MS reference method. In boys, the striking E2 peak during mini-puberty as well as high pubertal E2 without phenotypic estrogenization in regular male puberty indicates that the role of E2 in children and, especially in boys, requires better functional understanding.
Study context
- Population
- Healthy children aged 0–18 yearshealthy children
- Participants
- 1,181 vs 543
- Comparison
- Girls vs Boys
- Girlsn = 1,181
- Boysn = 543
- Outcome
- Serum E2 reference intervals, 0-18 years
Methodological detailsUPLC-MS/MS method development, systematic literature comparison
- UPLC-MS/MS method development
- Systematic literature comparison
Main finding
As reported in the abstract
- Lower limit of quantification
- 11 pmol/L
- E2 peak during mini-puberty, girls
- up to 192 pmol/L
- E2 peak during mini-puberty, boys
- up to 225 pmol/L
- Pubertal E2, girls (higher)
- up to 638 pmol/L
- Boys' reference intervals (RIs), overlapping considerably with girls'
- up to 259 pmol/L
Further findings
Literature comparison of LC-MS/MS pediatric E2 RIs
- Studies found using LC-MS/MS with RIs for complete pediatric age range
- 4
- Studies (including ours) documenting higher E2 during mini-puberty in both sexes
- 3
- RIs varied considerably; pre-pubertal and pubertal phases present in all studies
Original publication
New liquid chromatography tandem mass spectrometry reference data for estradiol show mini-puberty in both sexes and typical pre-pubertal and pubertal patterns. Eur J Endocrinol. Published 23 April 2024. doi:10.
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