Metabolic memory in diabetic foot syndrome (DFS): MICRO-RNAS, single nucleotide polymorphisms (SNPs) frequency and their relationship with indices of endothelial function and adipo-inflammatory dysfunction
Authors' conclusion
Our results highlighted that patients with diabetic foot showed predominant expression of the VEGF C2578A CC polymorphism and reduced expression of the AC allele. Additionally, we found an overexpression of miR-217-5p and miR-503-5p in diabetic patients with and without diabetic foot syndrome compared with healthy controls. These results align with those reported in the literature, in which the overexpression of miR-217-5p and miR-503-5p in the context of diabetic foot is reported. The identification of these epigenetic modifications could therefore be helpful in the early diagnosis of diabetic foot and the treatment of risk factors. However, further studies are necessary to confirm this hypothesis.
Study context
- Population
- Patients with diabetes with and without lower limb ulcers, and nondiabetic controls, enrolled March 2021–June 2022
- Participants
- 110 patients
- Exposure
- Diabetic patients with diabetic foot vs diabetic patients without ulcers vs nondiabetic controls
- Diabetic footn = 50
- Diabetes without ulcersn = 40
- Controlsn = 20reference
- Outcome
- Serum VEGF, HIF-1alpha and Gremlin-1
Main finding
As reported in the abstract
VEGF (pg/mL), diabetic foot vs without ulcers vs controls, mean ± SD
191.40 ± 200 vs. 98.27 ± 56.92 vs. 71.01 ± 52.96
p = 0.22
HIF-1alpha (ng/mL), diabetic foot vs without ulcers vs controls, mean ± SD
40.18 ± 10.80 vs. 33.50 ± 6.16 vs. 33.85 ± 6.84
p = 0.10
Gremlin-1 (ng/mL), diabetic foot vs without ulcers vs controls, mean ± SD
1.72 ± 0.512 vs. 1.31 ± 0.21 vs. 1.11 ± 0.19
p < 0.0005
Observational data: associations, not effects.
Further findings
| Outcome | Estimate | P value |
|---|---|---|
| miR-217-5p and miR-503-5p expression vs controlsDiabetic foot vs Controls · higher with Diabetic foot | fold expression miR-217-5p 2.19 | P < 0.05 |
| miR-217-5p and miR-503-5p expression vs controlsDiabetic foot vs Controls · higher with Diabetic foot | fold expression miR-503-5p 6.21 | P = 0.001 |
| miR-217-5p and miR-503-5p expression vs controlsDiabetes without ulcers vs Controls · higher with Diabetes without ulcers | fold expression miR-217-5p 2.41 | P = 0 |
| miR-217-5p and miR-503-5p expression vs controlsDiabetes without ulcers vs Controls · higher with Diabetes without ulcers | fold expression miR-503-5p 2.24 | P = 0.029 |
VEGF C2578A polymorphism, diabetic patients with and without ulcers vs controls
- Higher expression of VEGFC2578A CC polymorphism vs controls
- P = 0.001
- Lower expression of VEGFC2578A AC polymorphism vs controls
- P < 0.005
Original publication
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