Investigation of pH-dependent 1H NMR urine metabolite profiles for diagnosis of obesity-related disordering
Authors' conclusion
We concluded that 1H NMR-derived urine metabolite profile represents a snapshot that can reveal the physiological condition of humans in either a healthy or diseased state under intrinsic urine pH. We demonstrated a systematic analysis of pH-dependent modulations on the human urine metabolite signals and further developed software for quantification of urine metabolite profiles with high accuracy, enabling the uncovering of potential metabolite biomarkers in clinical diagnosis applications.
Study context
- Population
- Patients with Ob-HL and healthy controls; urine 1H NMR across urine pH 4.5 to 9.5
- Comparison
- Ob-HL vs healthy controls; pH-dependent NMR approach vs neutralizing pH approach
- Outcome
- Diagnostic accuracy of urine metabolites for discriminating Ob-HL from healthy controls
Methodological details1H NMR metabolomics, pH-dependent chemical shift database, "pH intelligence" program
- 1H NMR metabolomics
- pH-dependent chemical shift database
- "pH intelligence" program
Performance
As reported in the abstract
| Test or model | AUC |
|---|---|
| TMAO (pH-dependent approach) | 0.902 |
| Neutralizing pH approach | 0.549 |
AUC
TMAO (pH-dependent approach)0.902
Neutralizing pH approach0.549
- Potential biomarkers (TMAO, glycine, pyruvic acid) with VIP score >1.0 and q-value < 0.05
- 3
Original publication
Investigation of pH-dependent 1H NMR urine metabolite profiles for diagnosis of obesity-related disordering. Int J Obes (Lond). Published 10 December 2024. doi:10.
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