Digital quantification of somatostatin receptor subtypes 2 and 5 in growth hormone–secreting pituitary tumors
Authors' conclusion
The SSTR2 %PC correlated with treatment response only when evaluated using the DIA, showing a better performance of this method.
Study context
- Population
- Growth hormone (GH)-secreting pituitary tumors in acromegaly, new cohort, immunohistochemistry (IHC) performed in a different laboratory
- Participants
- 42 GH-secreting tumors
- Comparison
- Immunoreactivity score (IRS) vs digital image analysis (DIA) for SSTR2 and SSTR5 in predicting fg-SRL response
- Outcome
- SSTR2 expression predicting response to fg-SRL
Methodological detailsvalidation of open-source digital image analysis (CellProfiler)
- Validation of open-source digital image analysis (CellProfiler)
Performance
As reported in the abstract
| Test or model | Cut-off | AUC | Sensitivity | Specificity |
|---|---|---|---|---|
| IRS | ≥ 5 | 0.763 | 77% | 83% |
| Intensity/area | ≥0.106 | 0.833 | 92% | 83% |
| %PC-DIA | ≥63.7% | 0.917 | 92% | 83% |
Cut-off
IRS≥ 5
Intensity/area≥0.106
%PC-DIA≥63.7%
AUC
IRS0.763
Intensity/area0.833
%PC-DIA0.917
Sensitivity
IRS77%
Intensity/area92%
%PC-DIA92%
Specificity
IRS83%
Intensity/area83%
%PC-DIA83%
Further findings
| Outcome | P value |
|---|---|
| Correlation between IRS and DIA for SSTR2 and SSTR5IRS vs DIA · good correlation for both SSTR2 and SSTR5 | P < 0.001 |
fg-SRL response by SSTR subtype
- Response to fg-SRL correlated with SSTR2, but not SSTR5, expression
- SSTR2 %PC correlated with response only when evaluated by DIA
Original publication
Digital quantification of somatostatin receptor subtypes 2 and 5 in growth hormone–secreting pituitary tumors. Eur J Endocrinol. Published 21 December 2024. doi:10.
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