Development of an enzyme-linked immunosorbent assay for newborns dried blood spot thyroglobulin
Authors' conclusion
This DBS-Tg assay demonstrated good analytical performance over a wide range of Tg concentrations, suggesting it is well suited to detecting variations in Tg concentrations. Studies comparing populations with different prevalence of anemia should consider the effect of hematocrit on DBS-Tg determination. The availability of a DBS-Tg assay for newborns makes it possible to integrate iodine status monitoring with newborn screening for inherited metabolic diseases.
Study context
- Population
- Newborns delivered from healthy euthyroid pregnant women; cord blood
- Participants
- 209 newborns
- Comparison
- DBS-Tg enzyme-linked immunosorbent assay (ELISA) vs cord plasma thyroglobulin (Tg)
- Outcome
- Analytical performance of DBS-Tg assay
Methodological detailsELISA adapted for dried blood spots (DBS)
- ELISA adapted for DBS
Performance
As reported in the abstract
Limit of detection
2.4 µg/L
Limit of quantification
5.8 µg/L
Repeatability
7.7-8.3%
Intermediate fidelity
11.0-11.2%
Further findings
Median Tg in newborns — between DBS-Tg (ELISA) and Cord plasma Tg (ELISA) · not significantly different
P = 0.48
- Median Tg in newborns — Correlation (R)
- 0.88
Effect of hematocrit on DBS-Tg
- DBS-Tg concentrations decrease with increasing hematocrit (P < 0.05)
Stability of DBS-Tg · 12 months
- Stable at 25 µg/L for 12 months at −20ºC and 4ºC
Original publication
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