A Preliminary Analysis of Thyrotropin Measurement from Finger Stick Dried Blood Spot with an Automated High-Throughput Immunoassay Analyzer
Authors' conclusion
TSH from DBS may be accurately measured on an automated high-throughput immunoassay analyzer and could be used to diagnose hypothyroidism and, for the first time, hypothyrotropinemia.
Study context
- Population
- Patients presenting to the outpatient clinic of the University Medical Center Utrecht
- Participants
- 109 patients
- Comparison
- Finger stick DBS TSH vs venous plasma TSH
- Outcome
- Method comparison of TSH: venous plasma vs finger stick DBS
Methodological detailsmethod comparison, single-centre, Validation of stability, hematocrit (Hct) effect, precision, and limits of blank and quantitation per Clinical and Laboratory Standards Institute evaluation protocol
- Method comparison
- Single-centre
- Validation of stability, Hct effect, precision, and limits of blank and quantitation per Clinical and Laboratory Standards Institute evaluation protocol
Performance
As reported in the abstract
R2
0.988
95% CI 0.986–0.990
Correct diagnosis of hypothyrotropinemia
12 of 14
Correct diagnosis of hypothyroidism
6 of 7
Further findings
Stability and effect of Hct
- TSH from DBS stable at −20°C to +30°C for at least
- 4 days
- Maximum decrease of eluate TSH per 1% increase in Hct
- 1.13%
Original publication
A Preliminary Analysis of Thyrotropin Measurement from Finger Stick Dried Blood Spot with an Automated High-Throughput Immunoassay Analyzer. Thyroid. Published 15 March 2023. doi:10.
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