Circulating Small Noncoding RNA Profiling as a Potential Biomarker of Atherosclerotic Plaque Composition in Type 1 Diabetes
Authors' conclusion
These findings characterize miRNAs and their signature in the regulatory network of carotid plaque phenotype in T1D, providing new insights into plaque pathophysiology and possibly novel biomarkers of plaque composition.
Study context
- Population
- Subjects with T1D with carotid calcified or fibrous plaque; exploratory set 26 CCP and 25 CFP; validation set 40 CCP, 40 CFP and 24 controlsexploratory and validation sets
- Comparison
- Carotid calcified plaque vs carotid fibrous plaque
- Controln = 24
- Outcome
- Circulating miRNA expression profile
Methodological detailscase–control, exploratory and validation sets, next-generation sequencing, digital droplet PCR
- Case–control
- Exploratory and validation sets
- Next-generation sequencing
- Digital droplet PCR
Performance
As reported in the abstract
Differential circulating miRNA expression, CCP vs CFP
- miR-503-5p, let-7d-5p, miR-106b-3p and miR-93-5p significantly upregulated in CCP vs CFP
- miR-10a-5p downregulated in CCP vs CFP
- CCP vs CFP: P < 0.05
Further findings
Correlation of candidate miRNAs with LDL-C in CCP
- All candidate miRNAs significantly correlated with LDL-C in CCP: direct for upregulated, inverse for downregulated miRNA
Target genes of upregulated miRNAs in CCP
- Many target genes involved in osteogenic differentiation, apoptosis, inflammation, cholesterol metabolism and extracellular matrix organization
Original publication
Circulating Small Noncoding RNA Profiling as a Potential Biomarker of Atherosclerotic Plaque Composition in Type 1 Diabetes. Diabetes Care. Published 28 December 2022. doi:10.
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