Circulating miRNAs Detect High vs Low Visceral Adipose Tissue Inflammation in Patients Living With Obesity
Authors' conclusion
Circ-miRs that identify patients with low- vs high-VAT inflammation constitute a putative tool to improve personalized care of patients with obesity.
Study context
- Population
- Patients living with obesity undergoing bariatric surgery; Visceral adipose tissue (VAT) inflammation classified low/high by an expression score from TNFA, IL6 and CCL2 mRNA levels
- Participants
- 35 vs 51
- Comparison
- Circulating miRNAs combined with homeostatic model assessment of insulin resistance (HOMA-IR) to discriminate high vs low VAT inflammation
- Discoveryn = 35
- Validationn = 51
- Outcome
- Discriminative power of differentially expressed circ-miRs to detect low/high VAT inflammation (ROC-AUC)
Methodological detailsdiscovery and validation cohorts
- Discovery and validation cohorts
Performance
As reported in the abstract
ROC-AUC of circ-miRs 181b-5p, 1306-3p and 3138 combined with HOMA-IR for high vs low VAT inflammation
| Test or model | AUC |
|---|---|
| Discovery | 0.951 (95% CI, 0.865-1) |
| Validation | 0.808 (95% CI, 0.654-0.963) |
AUC
Discovery0.951 (95% CI, 0.865-1)
Validation0.808 (95% CI, 0.654-0.963)
Further findings
circ-miR screening in discovery cohort
- circ-miRs associated with high-VAT inflammation (Mann-Whitney)
- 53 out of 263 (20%)
- Differentially expressed by Deseq2
- 12 (12/53 = 23%)
- Discriminated high vs low VAT inflammation with receiver operating characteristic-area under the curve (ROC-AUC) >0.8
- 6
- Differentially abundant in all 3 approaches
- 5
- Unaffected by hemolysis and validated in independent cohort
- 3
Predicted target genes
- Enriched in pathways of insulin and inflammatory signaling, circadian entrainment and cellular senescence
Original publication
Circulating miRNAs Detect High vs Low Visceral Adipose Tissue Inflammation in Patients Living With Obesity. J Clin Endocrinol Metab. Published 15 September 2023. doi:10.
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