Changes in the gut bacteriome upon gluten-free diet intervention do not mediate beta cell preservation
Authors' conclusion
The bacteriome reacted to GFD, but the changes were unrelated to the pace of beta cell capacity loss. The previously observed moderately protective effect of GFD is therefore mediated through other pathways.
Study context
- Population
- Children (aged 10.2 ± 3.3 years) with recent-onset T1D
- Participants
- 45 children
- Comparison
- Gluten-free diet (GFD) vs control
- Primary endpoint
- GFD-associated changes in gut bacteriome composition and functional capacity, and whether they mediated effects of GFD on beta cell function
Methodological detailsstool samples at 3-month intervals, Bacteriome 16S rDNA profiles, PICRUSt2 functional prediction, NMR metabolomics; zonulin at 1, 6 and 12 months and lactulose/mannitol test at end
- Stool samples at 3-month intervals
- Bacteriome 16S rDNA profiles, PICRUSt2 functional prediction, NMR metabolomics; zonulin at 1, 6 and 12 months and lactulose/mannitol test at end
Primary result
As reported in the abstract
Gut bacteriome composition with GFD
- Bifidobacterium decrease with GFD (adjusted p=1.4 × 10-4 DESeq2; p=0.026 GEE)
- 3.3-fold
- Roseburia increase with GFD (adjusted p=0.02 DESeq2; p=0.002 GEE)
- 2.4-fold
- Neither genus change associated with pace of beta cell loss
- p>0.50
Further findings
Other taxa and beta cell loss
- Several Bacteroidaceae genera yielded suggestive signals
Faecal metabolome
- Metabolome ordination correlated with bacteriome but did not associate with GFD or beta cell preservation categories
Gut permeability
- No indication of changes in gut permeability
Original publication
Changes in the gut bacteriome upon gluten-free diet intervention do not mediate beta cell preservation. Diabetologia. Published 4 October 2022. doi:10.
Composed by AI from the published abstract and checked against it. How Briefs are made →