Spatial multiomics atlas reveals smooth muscle phenotypic transformation and metabolic reprogramming in diabetic macroangiopathy
Authors' conclusion
This study constructs the spatial gene-metabolism map of the whole anterior tibial artery for the first time and reveals the characteristics of vascular calcification, the phenotypic transformation trend of SMCs, and the transcriptional driving network of SMCs phenotypic transformation of diabetic macrovascular disease. In the perspective of combining the transcriptome and metabolome, the study demonstrates the activated metabolic pathways in diabetic blood vessels and the key genes involved in diabetic metabolic reprogramming.
Study context
- Population
- Diabetic patients with amputation; anterior tibial artery
- Participants
- 11 patients
Methodological detailssingle-cell transcriptome, spatial transcriptome, spatial metabolome
- Single-cell transcriptome
- Spatial transcriptome
- Spatial metabolome
Main finding
As reported in the abstract
- Spatial multiomics map of diabetic blood vessels constructed (single-cell and spatial transcriptome, spatial metabolome)
- Cell composition and biological function of calcified regions characterised by integrating spatial and single-cell omics
- SMCs can transform into pro-inflammatory chemotactic SMCs, macrophage-like/foam-like SMCs, and fibroblast/chondroblast SMCs
- SMC phenotypic transformation driven by transcription factor network incl. KDM5B, DDIT3
- HNMT and CYP27A1 participate in diabetic vascular metabolic reprogramming (with public data)
Original publication
Spatial multiomics atlas reveals smooth muscle phenotypic transformation and metabolic reprogramming in diabetic macroangiopathy. Cardiovasc Diabetol. Published 12 October 2024. doi:10.
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