Multiplexed Multiple Reaction Monitoring (MRM)-based proteomics for identification of circulating proteins as biomarkers of cardiovascular damage progression associated with diabetes mellitus
Authors' conclusion
Targeted mass spectrometry approach indicated 13 multiple circulating proteins as possible biomarkers of cardiovascular damage progression associated with T2DM, with excellent classification results in terms of sensitivity and specificity.
Study context
- Population
- Patients with T2DM and/or coronary heart disease (CHD); plasma samples
- Participants
- 34 / 31 / 30
- Exposure
- T2DM with CHD vs T2DM without CHD vs CHD without diabetes
- T2DM + CHDn = 34
- T2DM without CHDn = 31
- CHD without diabetesn = 30
- Outcome
- Predictive role of circulating proteins as biomarkers of cardiovascular damage progression associated with diabetes mellitus
Methodological detailstargeted liquid chromatography mass spectrometry (LC-MS) proteomics (MRM), multiplexed
- Targeted LC-MS proteomics (MRM)
- Multiplexed
Main finding
As reported in the abstract
Classification of the three groups by 13-protein panel (ROC, nominal logistic regression)
Proteins quantified in plasma
81
Proteins significantly different among groups (analysis of variance (ANOVA))
13
AUC range
0.91-0.97
Sensitivity and specificity range
85 to 100%
Observational data: associations, not effects.
Further findings
Cluster and principal component analysis of the 13 proteins
- First cluster: apolipoprotein C-III, C-II, A-IV, retinol-binding protein 4, lysozyme C, cystatin-C
- Second cluster: alpha 2 macroglobulin, afamin, kininogen 1, vitronectin, vitamin K-dependent protein S, complement factor B, mannan-binding lectin serine protease 2
Gene Ontology analysis
- Terms related to blood microparticles, extracellular exosomes and collagen-containing extracellular matrix
Original publication
Multiplexed MRM-based proteomics for identification of circulating proteins as biomarkers of cardiovascular damage progression associated with diabetes mellitus. Cardiovasc Diabetol. Published 20 January 2024. doi:10.
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