Gram-Negative Bacteria and Lipopolysaccharides as Risk Factors for the Occurrence of Diabetic Foot
Authors' conclusion
The skin microbiome in patients with diabetes undergoes dynamic changes at taxonomic and functional levels with the progression of diabetic complications. The increase in gram-negative bacteria on the skin surface through LPS-TLR4 signal transduction could induce inflammatory response in early diabetic skin lesions.
Study context
- Population
- Consecutive subjects stratified by diabetic foot (DF) risk, and healthy controls
- Participants
- 90 consecutive subjects
- Comparison
- Foot skin microbiome across DF risk strata vs healthy controls
- Outcome
- Foot skin microbiome composition and function
Methodological details16S rRNA and metagenomic sequencing, mouse model of microbiota transplantation
- 16S rRNA and metagenomic sequencing
- Mouse model of microbiota transplantation
Main finding
As reported in the abstract
- Proportion of gram-negative bacteria in plantar skin increased with progression of diabetic complications
- Moraxella osloensis a representative core strain in the high-risk group (metagenome, species level)
- Major microbial metabolites affecting diabetic skin lesions: increased amino acid metabolites; antibiotic resistance genes abundant
- Skin microbiota from high-risk patients induced more inflammatory cell infiltration (mouse), similar to lipopolysaccharide (LPS)-stimulated response, inhibited by TLR4 antagonists
Original publication
Gram-Negative Bacteria and Lipopolysaccharides as Risk Factors for the Occurrence of Diabetic Foot. J Clin Endocrinol Metab. Published 28 March 2023. doi:10.
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