Dysregulation of long non-coding RNA gene expression pathways in monocytes of type 2 diabetes patients with cardiovascular disease
Authors' conclusion
We identified dysregulated diabetes-related genes and pathways in monocytes of diabetes patients with cardiovascular complications, including lncRNA genes of unknown function strongly associated with networks of known diabetes genes.
Study context
- Population
- Patients with T2D (with cardiovascular disease (CVD), long-duration diabetes, poor glycaemic control) and non-diabetes controls; monocytes sorted from peripheral blood
- Participants
- 120 vs 37
- Exposure
- T2D vs non-diabetes controls; within T2D by CVD, diabetes duration and glycaemic control
- T2Dn = 120
- Non-diabetes controlsn = 37reference
- Outcome
- Differentially expressed lncRNAs in monocytes, T2D vs non-T2D
Methodological detailspaired-end RNA sequencing of monocytes, differential expression and co-expression analysis
- Paired-end RNA sequencing of monocytes
- Differential expression and co-expression analysis
Main finding
As reported in the abstract
Upregulated lncRNA ENSG00000287255
FDR = 0.017
Upregulated lncRNA ENSG00000289424
FDR = 0.048
Downregulated lncRNA ENSG00000276603
FDR = 0.017
Highest-scoring diabetes network-associated lncRNA (co-expression)
ENSG00000225822
UBXN7-AS1
Observational data: associations, not effects.
Further findings
lncRNAs within T2D by CVD
- lncRNA ENSG00000261654 upregulated in microvascular disease without clinically documented macrovascular disease
- FDR = 0.046
- lncRNAs strongly associated with diabetes networks, all patients with CVD combined
- ENSG0000028654, ENSG00000261326 (LINC01355), ENSG00000260135 (MMP2-AS1), ENSG00000262097, ENSG00000241560 (ZBTB20-AS1)
- Differentially expressed genes (DEGs) involved in networks affecting metabolic and cardiovascular pathologies
Co-expression lncRNAs, diabetes duration ≥ 10 years vs <10 years
- Two lncRNAs identified
- ENSG00000269019 (HOMER3-AS10) and ENSG00000212719 (LINC02693)
lncRNAs, good vs poor glycaemic control
- Two lncRNAs identified
- ENSG00000245164 (LINC00861) and ENSG00000286313
Original publication
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