Characterizing 24-Hour Skeletal Muscle Gene Expression Alongside Metabolic and Endocrine Responses Under Diurnal Conditions
Authors' conclusion
Diurnal rhythms were present in human skeletal muscle gene expression as well systemic metabolites and hormones under controlled diurnal conditions. The temporal patterns of genes relating to carbohydrate and lipid metabolism alongside circulating insulin are consistent with diurnal rhythms being driven in part by the diurnal influence of cyclic feeding and fasting.
Study context
- Population
- Healthy adults (9M/1F), mean ± SD age 30 ± 10 years, BMI 24.1 ± 2.7 kg·m-2, resting in the laboratory for 37 hours
- Participants
- 10 adults
- Outcome
- Temporal profiles of skeletal muscle expression of genes involved in carbohydrate metabolism, lipid metabolism, circadian clocks and autophagy, related to systemic metabolites and hormones
- Follow-up
- 24 hours (08:00-08:00 hours)
Methodological detailscontrolled laboratory protocol, hourly isocaloric liquid meal replacements, hourly blood sampling, muscle biopsies every 4 hours, Sleep opportunity 22:00 to 07:00 hours; biopsies from 12:00 to 08:00 hours the following day
- Controlled laboratory protocol
- Hourly isocaloric liquid meal replacements
- Hourly blood sampling
- Muscle biopsies every 4 hours
- Sleep opportunity 22:00 to 07:00 hours; biopsies from 12:00 to 08:00 hours the following day
Main finding
As reported in the abstract
24-hour rhythmicity (acrophase)
- Plasma insulin diurnal rhythmicity, peak
- 18:04 hours
- GLUT4 acrophase
- 14:40
- PPARGC1A acrophase
- 16:13
- HK2 acrophase
- 18:24
- FABP3 acrophase
- 12:37
- PDK4 acrophase
- 05:30
- CPT1B acrophase
- 12:58
- Circulating glucose diurnal rhythmicity
- 00:19 hours
- Nonesterified fatty acids diurnal rhythmicity
- 04:56
- Glycerol diurnal rhythmicity
- 04:32
- TG diurnal rhythmicity
- 23:14
- Urea diurnal rhythmicity
- 00:46
- CTX diurnal rhythmicity
- 05:07
- Cortisol diurnal rhythmicity
- 22:50
Original publication
Characterizing 24-Hour Skeletal Muscle Gene Expression Alongside Metabolic and Endocrine Responses Under Diurnal Conditions. J Clin Endocrinol Metab. Published 23 May 2024. doi:10.
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