Characterization of Immune Infiltrate Along the Leading Edge of Differentiated Thyroid Cancer
Authors' conclusion
Higher tumoral PD-1 and PD-L1 expression indicate their role in DTC occurrence. A trend for more Tregs and M2 macrophages but less M1 macrophages intratumorally in patients with distant metastatic DTC, suggests their potential role as prognostic biomarkers.
Study context
- Population
- Adults with differentiated thyroid cancer (DTC); thyroid tissue collected at thyroidectomy
- Participants
- 17 adults
- Comparison
- Tumor vs adjacent thyroid tissue from the same patient
- Outcome
- Mean percentage expression of immune markers (CD45, CD3, Tregs, CD4, CD8, CD68, M2 and M1 macrophages, PD-1, PD-L1), tumor vs adjacent tissue
Methodological detailsmultiplex immunofluorescence, paired comparison of tumor vs adjacent thyroid tissue, spatial analysis along tumor leading edge
- Multiplex immunofluorescence
- Paired comparison of tumor vs adjacent thyroid tissue
- Spatial analysis along tumor leading edge
Main finding
As reported in the abstract
Immune marker expression, tumor vs adjacent tissue
- PD-1 and PD-L1 showed a significant increase in expression intratumorally vs adjacent thyroid tissue
- Higher trend for M2 macrophages intratumorally vs adjacent tissue
- Along the leading edge, PD-L1 correlated negatively with CD45 and positively with CD163 intratumorally
- Tumor vs Adjacent thyroid tissue: P < 0.05
Further findings
Tumor marker expression by distant metastases (exploratory)
- Nonsignificant trend for higher FOXP3 but less CD8 and iNOS expression in DTC with (n = 3) vs without distant metastases (n = 14)
- Exploratory analysis
Tumor marker expression by thyroiditis (exploratory)
- Nonsignificant trend for higher CD58 and iNOS expression in DTC with (n = 7) than without thyroiditis (n = 10)
- Exploratory analysis
Original publication
Characterization of Immune Infiltrate Along the Leading Edge of Differentiated Thyroid Cancer. Thyroid. Published 12 July 2024. doi:10.
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