A hypoxia-derived seven-gene signature reflects immune remodeling in diabetic kidney disease: integrated transcriptomic analysis and experimental validation
Authors' conclusion
This hypoxia-associated seven-gene signature showed diagnostic potential and reflected immune and molecular heterogeneity in DKD.
Study context
- Population
- Renal transcriptomic datasets (GEO) of diabetic kidney disease (DKD) and control kidney samples
- Comparison
- DKD vs control kidney samples
- Outcome
- Internal discrimination of hypoxia-associated seven-gene signature between DKD and control kidney samples
Methodological detailsGEO renal transcriptomic datasets, WGCNA, SVM-RFE and LASSO regression, CIBERSORT and ssGSEA immune infiltration, validation in diabetic animal models, Nephroseq datasets and plasma RT-qPCR
- GEO renal transcriptomic datasets
- WGCNA, SVM-RFE and LASSO regression
- CIBERSORT and ssGSEA immune infiltration
- Validation in diabetic animal models, Nephroseq datasets and plasma RT-qPCR
Performance
As reported in the abstract
Seven-gene signature (EGF, CASP3, HDAC9, FN1, SERPINF1, VWF, NT5E): discrimination DKD vs control
AUC (95% CI 0.820-0.937), internal discrimination
0.879
Further findings
Molecular heterogeneity
- Score-defined subgroups and consensus clustering revealed distinct transcriptional and immune patterns
Experimental validation
- Animal models, external renal datasets and plasma RT-qPCR supported directionally consistent expression changes
Original publication
A hypoxia-derived seven-gene signature reflects immune remodeling in diabetic kidney disease: integrated transcriptomic analysis and experimental validation. Diabetes Res Clin Pract. Published 14 September 2026. doi:10.
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