The Significance of High Expression of Circulating miR-222-3p in Patients With Unilateral Primary Aldosteronism
Authors' conclusion
The present results demonstrated higher expression of circulating miR-222-3p in UPA than in BHA, providing a biochemical marker for subtype diagnosis of PA. Moreover, the correlation of miR-222-3p with adrenal cell proliferation and aldosterone secretion indicated its significant involvement in the pathogenesis of PA.
Study context
- Population
- Patients with primary aldosteronism (PA) (unilateral primary aldosteronism (UPA) or bilateral hyperaldosteronism (BHA))
- Comparison
- Circulating miRNA profiles: UPA vs BHA
- Outcome
- Circulating miR-222-3p expression
Methodological detailsdiscovery panel in matched cases followed by validation in larger sample, in vitro primary cultured adrenal cells
- Discovery panel in matched cases followed by validation in larger sample
- In vitro primary cultured adrenal cells
Main finding
As reported in the abstract
- miR-222-3p significantly higher in UPA than BHA, in adrenal veins and inferior vena cava
- miR-222-3p significantly higher in adrenal vein on tumor side vs nontumor side within UPA patients
Further findings
In vitro effects of miR-222-3p in primary cultured adrenal cells
- miR-222-3p mimics appeared to increase CYP11B2 expression (not statistically significant)
- miR-222-3p mimics significantly increased cell proliferation; inhibitors decreased it
- CDKN1B identified as predicted target gene of miR-222-3p
Correlations of miR-222-3p expression
- Significant positive correlation with tumor diameter and plasma aldosterone concentration after saline infusion test
Original publication
The Significance of High Expression of Circulating miR-222-3p in Patients With Unilateral Primary Aldosteronism. J Clin Endocrinol Metab. Published 18 July 2025. doi:10.
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